Composite

Part:BBa_K4575014:Design

Designed by: Sarah Hutzl, Sumohit Sharma   Group: iGEM23_Stuttgart   (2023-10-07)


Expression casette for Dextranase expression in Pichia pastoris


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3132
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 3644
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1832
    Illegal BsaI.rc site found at 1849
    Illegal BsaI.rc site found at 1942
    Illegal BsaI.rc site found at 2662


Design Notes

The Dextranase sequence was codon optimized for Pichia pastoris. The signalpeptide was removed from the Dextranase sequence while the propeptide sequence was kept.


Source

Synthesized by Integrated DNA Technologies. Genomic and synthetic sequences from various origins and sources (see basic part pages).

References